What is Src in cancer?

What is Src in cancer?

Src is a non-receptor tyrosine kinase that is deregulated in many types of cancer. Decades of research have revealed the crucial role of Src in many aspects of tumor development, including proliferation, survival, adhesion, migration, invasion and, most importantly, metastasis, in multiple tumor types.

What is viral src?

viral: 0.01 – 0.01 Mb. showSearch for. v-Src is a gene found in Rous sarcoma virus (RSV) that encodes a tyrosine kinase that causes a type of cancer in chickens. The src gene is oncogenic as it triggers uncontrolled growth in abnormal host cells. It was the first retroviral oncogene to be discovered.

What is the function of Src gene?

SRC is implicated in regulation of pre-mRNA-processing and phosphorylates RNA-binding proteins such as KHDRBS1 (Probable). Also plays a role in PDGF-mediated tyrosine phosphorylation of both STAT1 and STAT3, leading to increased DNA binding activity of these transcription factors (By similarity).

What is the function of Src kinase?

Src is a non-receptor protein tyrosine kinase that transduces signals that are involved in the control of a variety of cellular processes such as proliferation, differentiation, motility, and adhesion.

When was the SRC gene discovered?

Thus, by 1970, the viral src gene had been identified, although this name was only bestowed on it a few years later.

What does src bind?

The SH2 domain of Src family kinases consist of approximately 100 amino acids. This domain acts by binding to phosphorylated tyrosine residues. The strength of binding is dependent on the amino acids surrounding the phosphorylated tyrosine. The Src kinases Fyn, Src, and Yes all bind via their SH2 domains.

Is SRC a serine threonine kinase?

Src and Src-family protein-tyrosine kinases are regulatory proteins that play key roles in cell differentiation, motility, proliferation, and survival. Of the three protein-serine/threonine kinases, only phosphorylation by CDK1/cdc2 has been demonstrated to increase Src kinase activity.

What activates Src gene?

First, Src can be activated by receptor tyrosine kinases such as EGFR and HGF receptor (Maa et al., 1995; Mao et al., 1997). These receptors have been known to be active in the progression of cancer, and, in turn, may activate Src.

How does EGFR activate Src?

c-Src interacts with a number of receptor tyrosine kinases including EGFR. Activation of EGFR increases c-Src catalytic activity. c-Src, in turn, phosphorylates novel sites on EGFR, which promotes signaling downstream of the receptor. Phosphorylation of EGFR by c-Src has been shown to regulate EGF-induced mitogenesis.

How is V SRC activated?

Which antibody is used as the secondary antibody for SRC antibody?

Anti-mouse IgG, HRP-linked Antibody #7076 was used as the secondary antibody. Western blot analysis of extracts from various cell lines using Src Antibody. Immunoprecipitation of Src from A-431 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is Src Antibody.

What is sensitivity of SRC antibody?

Specificity / Sensitivity. Src Antibody detects endogenous levels of Src proteins. This antibody cross-reacts with over-expressed levels of Yes, Fyn, and Fgr but does cross-react with other Src family members.

What does the antibody SRC AA 487-536 detect?

This antibody detects endogenous levels of total Src protein. It will react with Src (pY529), Yes (pY538), and Fyn (Y531). Synthetic peptide corresponding to Human Src aa 487-536. WB: HEK-293, HeLa cells; mouse primary astrocytes. IHC-P: Human breast carcinoma tissue.

Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function.