What is the composition of Giemsa stain?
What is the composition of Giemsa stain?
Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains the parasite nucleus red, while the methylene blue component stains the cytoplasm blue. The thin film is fixed with methanol.
What is the use of Giemsa?
Uses of Giemsa Stain Giemsa stain is used to obtain differential white blood cell counts. It is also used to differentiate nuclear and cytoplasmic morphology of the various blood cells like platelets, RBCs, WBCs.
What is Giemsa powder?
Giemsa dye is a blend of several different dyes – Azure, Eosin, and Methylene Blue dyes. Giemsa dye is used for routine staining of blood smears and blood marrow samples. It is also used for identifying microorganisms, chromosomatic aberrations, visualization of chromosomes and mastocytes.
Is Giemsa acidic or basic?
Giemsa stain is based on the use of the Giemsa dye, a neutral blue dye consisting of an acid dye, eosin, and a basic dye, methylene azide, which is metachromatic (= which has the the property of giving certain fabrics a hue different from their own color).
How do you make a 5 Giemsa stain solution?
Recipe
- Dissolve 3.8g of Giemsa powder into 250ml of methanol.
- Heat the solution from step 1 to ~60oC.
- Slowly add in 250ml of glycerin to the solution from step 2.
- Filter the solution from step 3.
- The solution needs to stand a period of time prior to use.
Who invented Giemsa stain?
Gustav Giemsa was born in Germany in 1867, worked mainly as a chemist, and died in 1948.
How do you make a 5% Giemsa?
Popular Answers (1)
- Dissolve 3.8g of Giemsa powder into 250ml of methanol.
- Heat the solution from step 1 to ~60oC.
- Slowly add in 250ml of glycerin to the solution from step 2.
- Filter the solution from step 3.
- The solution needs to stand a period of time prior to use.
How do you prepare a solution of Giemsa stain?
Giemsa stain preparation
- Dissolve 3.8g of Giemsa powder within 250ml of methanol.
- Heat the solution at 60oC.
- Add 250ml of glycerin.
- Filter the solution.
- leave the solution to stand for about 1-2 months before use. Store the solution in a cool, dark place.
Which stain is used for malaria parasite?
Giemsa stain
The Giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. The classical staining procedure requires between 30 and 45 min.
How do you mix Giemsa solution?
Use undiluted Giemsa’s solution, stain slides with concentrated Giemsa solution for 15 min, followed by 10 sec washes with each of the following solutions: acetic acid (0.1%), distilled water, 2-Propanol (3 times). Slowly add 25 mL Giemsa’s solution to 275 mL buffer solution, mix and leave to stand for 10 min, and filter if necessary.
It is commonly used for G-banding (Giemsa-Banding) Giemsa stain is a differential stain and contains a mixture of azure, methylene blue, and eosin dye.
How long does it take for Giemsa to stain slides?
The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left to dry. Wikimedia Commons has media related to Giemsa stains.
How long does it take for Giemsa’s solution to stain spirochetes?
For spirochaetes (also known as spirochetes), diluted Giemsa’s solution can be used for 12- to 24-hour staining. Alternatively, staining time can be reduced to 6-8 hours by adding a small amount of potassium carbonate solution. Staining results are the same for either modification.
