What is the difference between TRIzol and QIAzol?

What is the difference between TRIzol and QIAzol?

I have researched about TRIzol and QIAzol for performing a isolation RNA from PBMCs, and studying the two protocols, I realized that both have basically the same composition (phenol/guanidine-based) and even the steps of the procedure in the protocols are very similar, but QIAzol is optimized to lysis tissues that have …

What is the difference between TRIzol and TRI reagent?

What does TRI Reagent® do? TRI Reagent® solution (also sold as TRIzol) is a mixture of a mixture of guanidine thiocyanate and phenol in a monophase solution that is used for the isolation of DNA, RNA and protein from biological samples of human, animal, plant, yeast, bacteria, and virus.

What is QIAzol?

The QIAzol Lysis Reagent is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of fatty tissues and inhibit RNases. We recommend cleanup of the redissolved RNA using RNeasy® Kits, which are based on silica-membrane technology, to remove any contaminating phenol.

What is the difference between TRIzol and TRIzol LS reagent?

The only difference between TRIzol Reagent and TRIzol LS Reagent is the concentration of components. TRIzol LS Reagent is slightly more concentrated. The formula allows lower quantities of reagent to be used relative to a liquid sample. (TRIzol = 10:1 required, TRIzol LS = 3:1 required).

What is QIAzol lysis reagent?

Product Details. QIAzol Lysis Reagent is optimized for lysis of fatty tissues. The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation.

Does QIAzol expire?

The QIAzol Lysis Reagent (cat. no. 79306) can be stored at room temperature (15–25°C) or at 2–8°C for at least 12 months.

Why TRIzol is called TRIzol?

TRIzol is a widely used chemical solution used in the extraction of DNA, RNA, and proteins from cells. TRIzol is the brand name of guanidinium thiocyanate from the Ambion part of Life Technologies, and Tri-Reagent is the brand name from MRC, which was founded by Chomczynski.

Why is TRIzol used in RNA extraction?

Overall, TRIzol® preserves the RNA quality, integrity and quantity, allowing it to double as a storage medium as well as an extraction/purification solution.

What is QIAzol Lysis Reagent?

How do you use Trizol for RNA extraction?

Remove the supernatant completely. Wash the RNA pellet once with 75% ethanol, adding at least 1 ml of 75% ethanol per 1 ml of TRIZOL Reagent used for the initial homogenization. Mix the samples by vortexing and centrifuge at no more than 7,500 x g for 5 minutes at 2 to 8 oC. Repeat above washing procedure once.

Does TRIzol affect qPCR?

Real-time PCR was used to compare qPCR master mixes. The results revealed that TRIzol and GENEzol obtained significantly higher yield of RNA (P<0.01) but that TRIzol had the highest contamination of protein and DNA (P<0.05).

Can TRIzol be frozen?

Any tissues to be extracted should be snap frozen in liquid nitrogen immediately after dissection. They can be stored indefinitely in a -80°C freezer until needed for RNA. Avoid allowing the sample to thaw prior to extraction.