What is the function of DPX?
What is the function of DPX?
DPX Mountant is a mixture of Distyrene, a plasticizer, and xylene. It is a colorless synthetic resin mounting media which replaces Xylene-Balsam. It preserves the stain and dries quickly.
Why DPX is used in mounting?
Adult tissue to be mounted in dibutyl phthalate in xylene (DPX) requires a second fixation prior to dehydration after staining is complete. The second fixation creates covalent bonds between the antibody and its antigen, which survive dehydration and preserve the antibody binding.
What is DPX mounting?
DPX Mountant for histology is a mixture of distyrene, a plasticizer, and xylene used as a synthetic resin mounting media, that replaces xylene-balsam. DPX Mountant dries quickly and preserves stain.
Is DPX Mountant soluble in Histoclear?
Hystomount, DPX, DePeX and Styrolite were found to be suitable. It was not possible to recommend any one of these in preference to the others. Histoclear is a non-toxic solvent which dissolves most xylene-based mountants as effectively as xylene itself. The use of this product is recommended.
What is the composition of DPX?
A mixture of distyrene (a polystyrene), a plasticizer (tricresyl phosphate), and xylene, called DPX, was introduced in 1939 and later modified by the substitution of a more satisfactory plasticizer, dibutylphthalate (butyl, phthalate, styrene – BPS).
Is important as a mounting medium?
The main purpose of mounting media is to physically protect the specimen; the mounting medium bonds specimen, slide and coverslip together with a clear durable film. The medium is important for the image formation as it affects the specimen’s rendition.
How do you mount a DPX?
METHOD
- Dehydrate samples for mounting in DPX by passing them through graded alcohols.
- Add a small drop of DPX to the specimen.
- Remove any excess mount with a paper towel.
- DPX will set almost immediately and the samples can then be observed or photographed.
What is the refractive index of DPX?
~ 1.520
Specifications
| Appearance (Clarity) | Clear |
|---|---|
| Appearance (Colour) | Colourless |
| Appearance (Form) | Viscous liquid |
| Density (g/ml) @ 20°C | 0.920 – 0.921 |
| Refractive Index (20°C) | ~ 1.520 |
What is DPX in full form?
Digital Picture Exchange (DPX) is a common file format for digital intermediate and visual effects work and is a SMPTE standard (ST 268-1:2014). …
How do I mount a DPX slide?
Does Histoclear remove wax?
7 The stains we use are water-soluble. As paraffin wax is immiscible with water, you must remove the wax by soaking the dry slides in Histoclear and gradually rehydrate the sections in decreasing concentrations of alcohol, as summarized in Table 1.
What is refractive index of DPX?
What is DPX mountant for histology?
DPX Mountant for histology is a mixture of distyrene, a plasticizer, and xylene used as a synthetic resin mounting media, that replaces xylene-balsam. DPX Mountant dries quickly and preserves stain. DPX Mountant is suitable for HE- (Hematoxylin-Eosin) and Masson-Goldner staining.
What is DPX used for?
DPX is suitable for all staining techniques which are compatible with the use of alcohol and aromatic (xylene/ toluene) clearing agent. DPX is clear and colorless and will not discolor with age. The formula contains an anti – oxidant to inhibit stain fading, and is completely soluble in xylene and toluene.
What is the Thermo Scientific™ DPX mounting media?
The Thermo Scientific™ DPX Mounting Media is used as a synthetic mounting medium in histology and cytology.
Is the DPX hit dagger a good idea?
Whether you need something to make you feel safe walking downtown, or a last ditch defense against a bear attack, having the DPx HIT Dagger at the ready is a great idea. DPX GEAR RELEASES A POPULAR PRICED HEST/F CLASSIC AND A LIMITED RUN DECADE EDITION – JUST IN TIME FOR CHRISTMAS.
