What is the function of Gal80?
What is the function of Gal80?
In the absence of galactose, Gal80 binds to and inhibits the transcriptional activation domain (AD) of the GAL gene activator, Gal4, preventing GAL gene expression. Galactose triggers an association between Gal3 and Gal80, relieving Gal80 inhibition of Gal4.
How does Gal80 inhibit Gal4?
In the absence of galactose, the Gal80 protein binds to a small peptide (amino acids [aa] 855 to 870) within the Gal4AD and thereby prevents Gal4-mediated promoter activation (10, 11). Galactose converts Gal3 to a form that readily binds to Gal80 (21).
What is Gal80?
The Saccharomyces cerevisiae GAL80 gene ( SGDID:S000004515 ) is a negative regulator of the Saccharomyces cerevisiae GAL4 transcriptional activator. GAL80 can potentially be used to suppress any binary expression driver or split system hemidriver that contains the interacting C-terminal portion of the GAL4 gene.
What happens when Gal3 binds to Gal80?
We propose that the binding of Gal3 to the Gal80 monomer competes with Gal80 self-association, reducing the amount of the Gal80 dimer available for inhibition of Gal4.
Is GAL80 an activator or inhibitor?
The Gal80 protein inhibits Gal4p-mediated transcription activation by binding to the transcription activation domain. Inhibition is relieved by formation of an alternative Gal80-Gal3 complex.
What regulates GAL80?
GAL80 encodes a transcriptional repressor involved in transcriptional regulation in response to galactose (2). GAL80 transcription is also regulated by the Gal3p/Gal80p/Gal4p feedback mechanism that regulates the other GAL genes.
Is Gal80 an activator or inhibitor?
What does GAL4 bind to?
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′.
What is the consequence of DNA methylation?
DNA methylation, a process of adding a methyl group to DNA done by a DNA methyltransferase is a heritable (epigenetic) alteration leading to cancer, atherosclerosis, nervous disorders (Imprinting disorders), and cardiovascular diseases.
How are miRNAs expressed?
Recent studies utilizing transcriptome analysis, including that of Chaulk and coworkers [34], showed that miRNAs are mostly clustered and expressed as a single pri-miRNA transcript. For example, miR-11 and miR-998 have recently been shown to be organized in a cluster and have a co-dependent expression pattern [35].
How can microRNAs miRNAs regulate gene expression quizlet?
How do microRNAs (miRNAs) regulate genes? miRNAs bind to mRNA and prevent translation. What specific role does small interfering RNA (siRNA) have in the formation of heterochromatin? siRNA binds to enzyme complexes and guides them to targeted DNA.
