What is the mechanism of action for doxorubicin?
Doxorubicin has antimitotic and cytotoxic activity through a number of proposed mechanisms of action: Doxorubicin forms complexes with DNA by intercalation between base pairs, and it inhibits topoisomerase II activity by stabilizing the DNA-topoisomerase II complex, preventing the religation portion of the ligation- …
How does doxorubicin adriamycin work?
Adriamycin (doxorubicin) belongs to a class of cancer medicines called anthracycline antibiotics. It works by damaging the DNA inside cancer cells so they cannot replicate. It works at any point in the cell cycle. Cyclophosphamide belongs to a class of cancer medicines called alkylating agents.
How does doxorubicin work in the body?
How doxorubicin works. Doxorubicin is a type of chemotherapy drug called an anthracycline. It slows or stops the growth of cancer cells by blocking an enzyme called topo isomerase 2. Cancer cells need this enzyme to divide and grow.
What is pegylated liposomal doxorubicin?
Pegylated liposomal doxorubicin (Doxil, Caelyx) is a formulation of doxorubicin in poly(ethylene glycol)-coated (stealth) liposomes with a prolonged circulation time and unique toxicity profile. We review the preclinical and clinical pharmacology as well as recent clinical data obtained in specific cancer types.
How does doxorubicin affect DNA methylation?
Herein, we show that the enzymatic activity of DNMT1, the primary DNA methyltransferase in mammalian cells, is inhibited by DNA intercalators, such as doxorubicin, in an in vitro assay. Enzymatic analyses indicate that doxorubicin inhibits the catalytic activity of DNMT1 via DNA intercalation.
How does doxorubicin affect cell cycle?
DOX treatment resulted in significant cellular morphological alteration with increased intracellular granularity and cell size. DOX inhibited cell proliferation through cell cycle arrest at the G(2)/M phase as well as by cell death.
What is the mechanism by which 5-FU selectively kills fast growing cells?
5-FU is the backbone substance of advanced gastric cancer chemotherapy (5). 5-FU kills tumor cells through misincorporation of fluoronucleotides into DNA and RNA molecules and by inhibition of the nucleotide synthesis enzyme thymidylate synthase (TS) (6).