What is the purpose of the oxidation with periodic acid in the PAS staining protocol?
What is the purpose of the oxidation with periodic acid in the PAS staining protocol?
This stain is used for the demonstration of glycogen. Tissue sections are first oxidized by periodic acid. The oxidative process results in the formation of aldehyde groupings through carbon-to-carbon bond cleavage. Free hydroxyl groups should be present for oxidation to take place.
What is the significance of a positive periodic acid-Schiff PAS stain?
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
What is the purpose of periodic acid in this stain?
Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues.
What is the principle of periodic acid-Schiff?
PRINCIPLE: The PAS stain is a histochemical reaction in that the periodic acid oxidizes the carbon to carbon bond forming aldehydes which react to the fuchsin-sulfurous acid which form the magenta color. CONTROL: For staining fungus; use a known positive such as those used for the GMS.
What is the staining procedure of PAS stain?
Procedure for Periodic Acid-Schiff (PAS) Staining Rinsing: In distilled water. Aldehydration: Place the stain in Schiff reagent for 15 minutes, which turns light pink. Washing: Using lukewarm tap water, wash the stain for 5 minutes, turning it dark pink. Counterstaining: Add Mayer’s Hematoxylin for 1 minute.
How do you make a Schiff solution?
Schiff’s Reagent
- Dissolve 5g of basic fuchsin in 900ml of boiling distilled water.
- Cool to approximately 50oC and slowly add 100ml of 1N HCl.
- Cool to approximately 25oC and dissolve 10g of K2S2O5
- Shake for 3 minutes and incubate in the dark at room temperature for 24 hours.
What is PAS stain used for?
PAS (Periodic Acid Schiff) Staining Protocol. NovaUltra Special Stain Kits. Description: This method is used for detection of glycogen in tissues such as liver, cardiac and skeletal muscle on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well.
Can periodic acid‑Schiff staining detect induced hepatocytes in human hepatic cells?
A previous induction method effectively induced differentiation and metabolic abilities in HPCs. Periodic acid‑Schiff (PAS) staining is used to identify glycogen synthesis and hepatocyte function; however, this method failed to detect induced hepatocytes.
What is the protocol for glycogen staining?
PAS (Periodic Acid Schiff) Staining Protocol Used for the detection of glycogen in tissues such as liver, cardiac and skeletal muscle on formalin-fixed paraffin-embedded tissue sections, and may be used for frozen sections as well. Solutions and Reagents: 0.5% Periodic Acid Solution
What happens when periodic acid reacts with Schiff’s reagent?
Periodic acid acts as oxidizing agent which oxidizes compounds having free hydroxyl group (-OH group) or amino/alkylamine group resulting in dialdehydes. These dialdehydes when exposed to Schiff’s reagent, an insoluble magenta colored complex is formed.
