What is TRE3G promoter?
What is TRE3G promoter?
TRE-3G promoter(PTRE3G) is a kind of eukaryocyte cell promoters that will be switched on after combine with the compound of Tet-On 3G(BBa_K1431101) protein and doxycycline(Dox) mix. The inducible promoter TRE3G provides for very low basal expression and high maximal expression after induction (Loew et. al., 2010).
How does Tet-on system work?
Tet-On system: tetracycline binds the rtTA transcription factor and allows it to bind DNA at the promoter. Gene expression is induced in the presence of tetracycline.
What is bidirectional promoter?
A bidirectional promoter is the intergenic region between two divergent genes located on complementary strands of the DNA which drives their coordinated transcription in opposite directions.
What is EFS promoter?
EF1alpha (or its short, intron-less form, EFS) is a promoter that has been recently used in many clinical trials. It is a cellular-derived enhancer/promoter with decreased cross-activation of nearby promoters, therefore hypothetically decreasing the risk of genotoxicity.
How does rtTA work?
Like tTA, rtTA is a fusion protein comprised of the TetR repressor and the VP16 transactivation domain; however, a four amino acid change in the tetR DNA binding moiety alters rtTA’s binding characteristics such that it can only recognize the tetO sequences in the TRE of the target transgene in the presence of the Dox …
Are all promoters bidirectional?
Nevertheless, the diversity of the applied approaches shows that transcriptional initiation at most, if not all, promoters occurs in a bidirectional manner, even if the final transcription products are mainly observed in one orientation.
What is bidirectional genes?
Bidirectional gene pairs, also called head-to-head gene pairs, are defined as two genes on different strands with adjacent 5′-ends. They are transcribed divergently in a variety of coordinated fashion. Furthermore, it is of particular interest to know when and how the bidirectional gene arrangement evolved.
What is a U6 promoter?
U6 is a type III RNA polymerase III promoter commonly used for driving small hairpin RNA (shRNA) expression in vector-based RNAi. In the design and construction of viral vectors, multiple transcription units may be arranged in close proximity in a space-limited vector.
How is tre3g activated in human cells?
P TRE3G retains very strong induced expression when activated by transactivator binding. The Tet-On 3G transactivator, which binds to and activates expression from TRE promoters in the presence of doxycycline (Dox). The Tet-On 3G system is optimized for expression in human cells and for sensitivity to Dox. Video Player is loading.
What does the tre3g and ef1α promoter do?
The first promoter, the TRE3G promoter, controls the expression of elements inserted into the multiple cloning site (MCS) located downstream of the TRE3G promoter. The second promoter, a constitutive EF1α promoter, controls expression of a resistance gene for drug blasticidin (Bsd) and the Tet-On 3G transactivator protein (Fig. 1a ).
What is Tet-on 3G inducible gene expression?
Discover Tet-On 3G inducible gene expression: learn about the technology, applications, choosing the right system for your application, and available products. The inducible P TRE3G promoter, which provides the lowest basal expression of any tetracycline-inducible promoter in the absence of bound transactivator.
What is the difference between P tre3g and Tet-on 3G?
The P TRE3G promoter contains mutations that reduce background expression by 5–20 fold compared to our previous tightest promoter, P Tight. The Tet-On 3G transactivator contains mutations that significantly increase its sensitivity to Dox.
