What type of genome does adenovirus have?
What type of genome does adenovirus have?
The adenovirus genome is a single linear molecule of double-stranded DNA (26 163–48 395 bp) containing an inverted terminal repeat (ITR) of 30–371 bp at its termini, with the 5′ ends of each DNA strand linked covalently to a virus-coded terminal protein (TP). The nucleotide composition is 33.6–67.6% G + C.
How many genes does HCMV have?
The low-passage strain Merlin has become the reference strain for wild-type HCMV, and its sequence entry (NCBI GenBank accession NC_006273, Table 1) currently contains a set of 170 genes.
Are adenoviruses Retroviruses?
Adenovirus is a double-stranded DNA virus that does not go through an RNA intermediate, and thus inserted sequences need not be compatible with transcription of the complete viral genome and its subsequent reverse transcription as for retroviral vectors.
Are adenoviruses RNA viruses?
Adenovirus infection, as is the case with other viruses, could lead to the production of double-stranded RNA (dsRNA) through symmetrical transcription of the viral genome.
What type of genome does Epstein Barr virus have?
The EBV genome is composed of linear double-stranded DNA, approximately 172 kilobase pairs (kb) in length. EBV has a series of 0.5 kb terminal direct repeats (TRs) and internal repeat sequences (IRs) that divide the genome into short and long, largely unique sequence domains (Fig. 27.1(a)).
What is the difference between helical and icosahedral viruses?
A helical virus is a virus that has a capsid shaped in a filamentous or rod-shaped structure that has a central cavity that encloses its nucleic acid. An icosahedral virus is a virus consisting of identical subunits that make up equilateral triangles that are in turn arranged in a symmetrical fashion.
Why is ex vivo gene therapy better?
The advantage of ex vivo gene therapy is the ability to characterize the genetically modified cells extensively and select them for desired phenotypes before using them for therapy. The property of the cells may also be chosen to enhance the therapeutic potential.
What is the function of the BZLF1 factor?
BZLF1 is a pioneer factor that reverses epigenetic silencing of viral DNA to allow escape from latency. Genome-wide analyses of Zta binding to the Epstein-Barr virus genome reveals interactions in both early and late lytic cycles and an epigenetic switch leading to an altered binding profile.
Can BZLF1 induce lytic viral gene expression and replication?
The authors demonstrate that lytic viral gene expression and replication can be induced by BRLF1, but not BZLF1, expression in an epithelial cell line (NOKs-Akata) in which lytic viral gene promoters remain largely unmethylated. BZLF1 is a pioneer factor that reverses epigenetic silencing of viral DNA to allow escape from latency.
Are BZLF1 gene polymorphisms associated with malignant lymphoma in pediatric patients?
Studied the effects of BZLF1 on cellular response to DNA damage in nasopharyngeal carcinoma cells. This is the first report to characterize BZLF1 gene polymorphisms in paediatric patients from our geographical region and to suggest the association of these polymorphisms with malignant lymphomas.
How do hhv8-rta and ebv-bzlf1 interact in dually-infected Pels?
HHV8-RTA and EBV-BZLF1 are co-localized and physically interact with each other in dually-infected PELs, required for the mutual inhibition of the two molecules Zta interaction with mtSSB serves the dual function of facilitating viral and blocking mitochondrial DNA replication.
