What type of media is a TSA plate?
What type of media is a TSA plate?
TSA is the medium specified as Soybean-Casein-Digest Agar Medium by the United States Pharmacopeia (USP) for the total aerobic count portion of microbial limit testing procedures. The medium is also used for maintenance of stock cultures, plate counts and isolation of organisms from a variety of specimen types.
Does TSA plate contain agar?
TSA plates are non-selective and non-differential plates. Contains 2.0% (20 g/L) soytone, 0.5% (5 g/L) sodium chloride, and 1.5% agar.
Is TSA defined media?
Glucose Salts Agar (GSA): is a simple, defined medium. Only organisms that can make all their cellular components from glucose and inorganic salts are able to grow on this medium. Tryptic Soy Agar (TSA): is a rich, undefined medium containing products of an enzymatic digest of protein and soy product.
Is TSA a complex media?
Tryptic Soy Agar (TSA) is a complex medium for cultivation and isolation of a wide range of bacteria, yeasts and molds. This medium contains several peptones, providing amino acids and polypeptides to ensure growth of a variety of microorganisms. Sodium chloride maintains osmotic equilibrium in the medium.
What is TSA agar?
TSA contains enzymatic digests of casein and soybean meal, which provide amino acids and other nitrogenous substances, making it a nutritious medium for a variety of organisms. Glucose is the energy source. Sodium chloride maintains the osmotic equilibrium, while dipotassium phosphate acts as buffer to maintain pH.
Is TSA enriched media?
Enriched Tryptic Soy Agar (ETSA) is an enriched, non-selective, and differential medium intended for the isolation, quantitation, and partial identification of many fastidious and non-fastidious microorganisms.
How do I make TSA media?
Preparation and Method of Use of Tryptic Soy Agar
- Suspend 45 grams in 1000 ml distilled water.
- Heat to boiling to dissolve the medium completely.
- Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.
- Cool to 45-50°C.
- Mix well and pour into sterile Petri plates.
Is TSA media selective or differential?
Tryptic Soy Agar (TSA): a general purpose, non-selective, non-differential, supportive medium that supports growth of all microorganisms that do not require special nutrients. Culture specimens may contain many different microorganisms.
