When should I use silver stain?
When should I use silver stain?
In pathology, silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels.
How much protein do you need for silver staining?
100 ng protein for CBB R-250, 30-40 ng for G-250 and 5-10 ng for Silver staining.
What is the purpose of silver staining?
Silver staining is the most sensitive colorimetric method for detecting total protein. The technique involves the deposition of metallic silver onto the surface of a gel at the locations of protein bands. Silver ions (from silver nitrate in the staining reagent) interact and bind with certain protein functional groups.
What is the minimum concentration of protein detected using CBB?
3-10 microgram of protein can be detected usually within 5 minutes in Coomassie stains. With additional water-based de-staining, as little as 7 ng of protein (BSA) can be detected.
Which protein staining method is best for small amounts of protein faint bands )?
silver staining
In case if your protein is not abundant, go for colloidal CBB staining (which can visualise as little as 20 ng) or silver staining if you want to just see the band. All the best.
What are the sensitivity limits for protein detection in CBB staining and silver staining?
It has a detection limit of ~ 0.1–0.5 μg protein, sensitive enough for most daily needs. Silver staining has greater sensitivity, but involves many more steps and solutions (see Silver Staining of SDS-polyacrylamide Gel).
How do you use Sypro Ruby protein gel stain?
Use SYPRO Ruby Protein Gel Stain for the visualization of proteins in both non-denaturing and denaturing gels, including 2-D electrophoresis. This stain has a higher sensitivity than many other protein stains, ideal for detection of difficult-to-stain proteins such as glycoproteins and lipoproteins.
What types of fluorescent protein Gel stains does Biobio-Rad offer?
Bio-Rad offers three types of fluorescent protein gel stains, each optimized for your imager requirements: Oriole™ fluorescent gel stain — An easy-to-use, fast, sensitive, and highly linear protein gel stain for visualization and quantitation of proteins in SDS-PAGE gels.
What is the best gel stain for protein electrophoresis?
SYPRO Ruby protein gel stain — The most widely used gel stain for 2-D electrophoresis, ready-to-use SYPRO Ruby protein gel stain is compatible with mass spectrometry and Edman-based sequencing applications. It can be visualized with UV or blue light transilluminators or laser scanners.
How long does it take to stain a protein sample?
The simple protocol requires a one-step staining process without prior fixing. Since destaining is not required, protein samples can be accurately visualized and quantitated in less than 2 hours. The excitation and emission characteristics of Oriole make it ideal for UV-based imagers.
