Why does carotene have a high Rf value?
Why does carotene have a high Rf value?
The Rf value of the xanthophyll is two thirds that of carotene because xanthophyll has an H bond with cellulose, which slows it down, and makes it less soluble in the solvent. Carotene is more soluble in 9:1 petroleum-ether acetone solution, which caused it to be carried higher and have a larger Rf value.
What is the Rf value of beta carotene?
0.90
The appointed Rf value for β-carotene was 0.90.
Why does carotene travel faster on chromatography paper?
Carotene moves the farthest because it is the most nonpolar of the pigments and it is attracted more strongly to the acetone-ligroin mixture (mobile phase) than to the paper. This stronger, nonbonded interaction with the mobile phase indicates that carotene is the most nonpolar pigment found in spinach chloroplasts.
Why do chlorophylls have lower Rf values than the carotenes?
The Rf value for chlorophyll is lower than the Rf value of carotene because the movement of chlorophyll is very little compared to carotene. Chlorophyll is a polar compound while carotene is a less polar compound.
Why is carotene more non polar than lycopene?
β-carotene will elute first because lycopene has more double bonds so it sticks to the column more readily than β-carotene, and therefore takes longer to move down the column. β-carotene is less polar than lycopene because it has less conjugated pi bonds.
Why carotene traveled the farthest distance?
The pigment that traveled the furthest was carotene xanthophyll because it was the most soluble in the solvent. Chlorophyll b traveled least because it was the least soluble in the solvent.
What does the RF value Show?
In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify compounds. The Rf value of a compound is equal to the distance traveled by the compound divided by the distance traveled by the solvent front (both measured from the origin).
Why is carotene nonpolar?
The structure of beta carotene is shown below. It only contains C-H, C-C, and C=C bonds which are all nonpolar. This means that beta carotene is a nonpolar compound.
What is Rfrf value in chromatography?
RF value (in chromatography) The distance travelled by a given component divided by the distance travelled by the solvent front. For a given system at a known temperature, it is a characteristic of the component and can be used to identify components. Click to see full answer Just so, what does Rf value mean in chromatography?
Why does xanthophyll have a higher Rf value than carotene?
The Rf value of the xanthophyll is two thirds that of carotene because xanthophyll has an H bond with cellulose, which slows it down, and makes it less soluble in the solvent. Carotene is more soluble in 9:1 petroleum-ether acetone solution, which caused it to be carried higher and have a larger Rf value.
What is the range of Rf values for pigments?
Now look at the Rf values, which range between 0 and 1, with 0 being a pigment that does not move at all, and 1 indicating a pigment that moves the same distance as the solvent.
What is the Rf value of the dye?
The Rf value is defined as the ratio of the distance moved by the solute (i.e. the dye or pigment under test) and the distance moved by the the solvent (known as the Solvent front) along the paper, where both distances are measured from the common Origin or Application Baseline, that is the point where the sample is
