Can I use neomycin instead of G418?
Can I use neomycin instead of G418?
Bottom line: Use G418 for single-selection experiments on eukaryotic cells. Use neomycin or kanamycin for single-selection experiments on prokaryotic cells where you are selecting for neomycin resistance genes. Kanamycin should be used over neomycin if elimination of Mycoplasma species from culture is necessary.
Is gentamicin and G418 the same?
Commonly know as G418 or G-418, Gibco Geneticin reagent is an aminoglycoside related to gentamicin, and is commonly used as a selective agent for eukaryotic cells. As an analog of neomycin sulfate, it interferes with the function of 80S ribosomes and protein synthesis in eukaryotic cells.
How long does G418 take to kill cells?
3. Replace the G418-containing medium every 2 – 3 days and examine cells for visual toxicity. Most non-transfected (non-resistant) cells will die within 10 days, leaving the transfected cells to expand.
What concentration of neomycin is used for selection?
Bacterial selection antibiotics
| Selection antibiotic | Most common selection usage | Common working concentration |
|---|---|---|
| Mycophenolic Acid | Mammalian and Bacteria | 25 µg ⁄ ml |
| Neomycin Sulfate | Bacteria | 50 µg ⁄ ml |
| Polymyxin B Sulfate | Bacteria | 100 units / ml |
| Puromycin | Eukaryotic and Bacteria | 0.2 – 5 µg ⁄ ml |
What is G418 sulfate?
G418 also known as Geneticin® is an aminoglycoside antibiotic related to Gentamicin. Used as a selective agent in transfection of eukaryotic cells. Has highest potency ≥730 µg/mg and purity ≥98%.
What are selection antibiotics?
There is a long list of antibiotics available to researchers when applying selective pressure in the creation of a stable cell line. The choices include zeocin, hygromycin, blasticidin, puromycin and geneticin (G418). Researchers use different antibiotics due to cost or availability in their lab.
Is G418 light sensitive?
Sensitive to light. Optimal solvent is ethanol. Geneticin (G418) is an aminoglycoside antibiotic similar in structure to gentamicin B.
How do you administer gentamicin?
Gentamicin injection comes as a liquid to be injected intravenously (into a vein) or intramuscularly (into a muscle). When gentamicin is injected intravenously, it is usually infused (injected slowly) over a period of 30 minutes to 2 hours once every 6 or 8 hours.
What is G418 used to select for?
Geneticin (G418) is used to select for cells expressing neomycin resistance gene. b. Passaging cells frequently may speed up blasticidin selection.
What is the mechanism of action of G418?
G418 also known as G-418, G418 sulfate or Geneticin is an aminoglycoside antibiotic produced by Micromonospora rhodorangea . G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells . Resistance to G418 is conferred by the Neomycin resistance gene (neo)…
Can G418 be used in 293T cells?
Michel Ouellet is correct. G418 will not work in 293T, but works well in 293 at 0.4 mg/ml as Elizabeth Johnstone suggested. If you plan to stick to 293T cells and need a different antibiotic marker, I suggest using puromycin. It doesn’t take as long to kill off untransfected cells. Can you help by adding an answer?
What is the optimal concentration of G418 for selection?
G418 is normally used at a concentration of 400 μg/ml. However, the optimal concentration needs to be determined for your cells. Suggested concentrations of G418 for selection in some examples of mammalian cells are listed below:
