What is a gateway entry vector?
What is a gateway entry vector?
Gateway vectors contain modified versions of the att sites so that scientists can easily clone in their desired DNA sequences. This reaction is catalyzed by the BP Clonase enzyme mix and generates the entry clone containing the DNA of interest flanked by attL sites.
What is the purpose of Gateway cloning?
Gateway® Cloning is a universal cloning technique developed by Invitrogen life technologies. Gateway® Cloning Technique allows transfer of DNA fragments between different cloning vectors while maintaining the reading frame. It has effectively replaced the use of restriction endonucleases and ligases.
What is the advantage of Gateway cloning?
One of the main advantages of the GATEWAY Cloning Technology is that once you have made an Entry Clone the gene of interest can be easily subcloned into a wide variety of Destination Vectors using the LR Reaction (see Figure 3).
What are ATT sites?
Site-specific integrative recombination of bacteriophage λ involves unequal partners. The minimal phage att site is composed of approximately 240-base pairs and four distinct binding sites for Int protein, at least three of which are crucial for function.
What is a destination vector?
Gateway Destination vectors are similar to classical expression vectors containing multiple cloning sites, before the insertion of a gene of interest, using restriction enzyme digestion and ligation. Gateway Destination vectors are commercially available from Invitrogen, EMD (Novagen) and Covalys.
What is the meaning of Gateway in computer?
1. A computer that sits between different networks or applications. The gateway converts information, data or other communications from one protocol or format to another. An Internet gateway can transfer communications between an enterprise network and the Internet. …
Is Gateway cloning reversible?
Invitrogen Gateway recombination cloning uses a one hour reversible recombination reaction, without using restriction enzymes, ligase, subcloning steps, or screening of countless colonies, thereby saving you time, money, and effort.
What is a gateway cassette?
A Gateway® cassette containing attR recombination sites flanking a ccdB gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway® vectors containing the ccdB gene.
What is Gateway Destination?
A tourist gateway (sometimes called a “Tourism Gateway” or “Gateway City”) is a place or settlement through which tourists typically first visit on their way to a tourist attraction or tourism region. Tourist gateways may not offer significant attractions themselves.
What is a gateway device?
Put simply, a gateway is a device that combines the functions of a modem and a router. A modem is a device that connects to your cable or phone lines and translates the data or information from the Internet to your computer or connected device.
What are gategateway-compatible destination vectors?
Gateway-compatible destination vectors contain the Gateway recombination sites, allowing rapid, efficient transfer of sequences into a variety of expression systems using site-specific recombination.
Why choose a gateway vector for protein expression?
Destination vector selection guides Gateway cloning technology is especially noted for its utility in protein expression. The flexibility and diverse selection of Destination vectors and host systems is particularly attractive for multidisciplinary protein expression studies. Destination vectors for protein expression
What is the gateway technology?
The Gateway Technology is a universal cloning method that takes advantage of the site-specific recombination properties of bacteriophage lambda (Landy, 1989) to provide a rapid and highly efficient way to move your gene of interest into multiple vector systems.
What is destdestination vectors?
Destination vectors selection tool Destination vector selection guides Gateway cloning technology is especially noted for its utility in protein expression. The flexibility and diverse selection of Destination vectors and host systems is particularly attractive for multidisciplinary protein expression studies.
