Why is Matrigel important?
Why is Matrigel important?
Numerous cell types require a surface for attachment to grow and proliferate. A gelatinous protein mixture derived from mouse tumor cells and commercialized as Matrigel is commonly used as a basement membrane matrix for stem cells because it retains the stem cells in an undifferentiated state.
Do you need to dilute Matrigel?
To prevent incomplete gel formation for in vivo applications, do not dilute Corning Matrigel matrix to a final concentration below 4 mg/mL.
What is Matrigel used for in research?
Matrigel has been widely used to study tumor cell invasion, and an altered extracellular matrix has been shown to promote tumorigenesis [16].
How is Matrigel produced?
Corning Matrigel matrix is a reconstituted basement membrane extracted from EHS mouse tumor. When the material is extracted from the tumor, it contains laminin, collagen IV, entactin, heparan sulfate proteoglycan, and growth factors that occur naturally in the EHS tumor.
How many growth factors are in Matrigel™?
Matrigel™ contains multiple growth factors (0-0.1 pg/ml basic fibroblast growth factor; 0.5-1.3 ng/ml epidermal growth factor; 15.6 ng/ml insulin-like growth factor-I; 12 pg/ml platelet derived growth factor; <0.2 ng/ml neuronal growth factor; and 2.3 ng/ml transforming growth factor-B).
What is Corning Matrigel matrix?
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
What is Matrigel in cell culture?
Matrigel: a complex protein mixture required for optimal growth of cell culture
Is Matrigel a well-defined basement membrane matrix?
A gelatinous protein mixture derived from mouse tumor cells and commercialized as Matrigel is commonly used as a basement membrane matrix for stem cells because it retains the stem cells in an undifferentiated state. However, Matrigel is not a well-defined matrix, and therefore can produce a source of variability in experimental results.
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