What is the processivity of DNA polymerase?
What is the processivity of DNA polymerase?
Processivity is defined as the ability of DNA polymerase to carry out continuous DNA synthesis on a template DNA without frequent dissociation. It can be measured by the average number of nucleotides incorporated by a DNA polymerase on a single association/disassociation event.
What is the processivity of DNA polymerase 3?
DNA polymerase III has a high processivity and therefore, synthesizes DNA very quickly. This high processivity is due in part to the β-clamps that “hold” onto the DNA strands.
Is DNA polymerase and exonuclease?
DNA polymerase I is a single polypeptide chain with 928 amino acids and molecular weight of 109 kDa. This exonuclease activity is called the proofreading or editing function of DNA polymerase I. Pol I also has a unique 5′ to 3′ exonuclease activity that is required for its DNA repair function.
What do you mean by processivity?
In molecular biology and biochemistry, processivity is an enzyme’s ability to catalyze “consecutive reactions without releasing its substrate”. For example, processivity is the average number of nucleotides added by a polymerase enzyme, such as DNA polymerase, per association event with the template strand.
How is processivity measured?
Processivity was calculated by determining the ratio between fluorescent soluble products, released by initial cuts at the reducing end, and nonfluorescent products released by subsequent processive steps.
What is DNA polymerase III’s function in DNA replication?
The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis. Together with a DNA helicase and a primase, Pol III HE participates in the replicative apparatus that acts at the replication fork.
What is the role of DNA exonuclease?
Exonucleases can act as proof-readers during DNA polymerisation in DNA replication, to remove unusual DNA structures that arise from problems with DNA replication fork progression, and they can be directly involved in repairing damaged DNA.
What is the difference between an endonuclease and an exonuclease?
The main difference between these enzymes is that endonucleases cleave the phosphodiester bond in the polynucleotide present internal in the polynucleotide chain, whereas exonucleases cleave the phosphodiester bond from the ends.
What is the difference between endonuclease and exonuclease?
What is meant by exonuclease and endonuclease?
Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain. Its close relative is the endonuclease, which cleaves phosphodiester bonds in the middle (endo) of a polynucleotide chain.
