What is a Superose 6 column?
What is a Superose 6 column?
Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.
What gel is used in gel filtration?
Gel Filtration Chromatography Media Commonly used media for gel filtration chromatography are based on agarose or polyacrylamide beads, dextrose for gravity or low-pressure systems, and polymeric resins for medium-pressure systems.
What is Sephadex gel filtration?
Sephadex is a cross-linked dextran gel used for gel filtration. By varying the degree of cross-linking, the fractionation properties of the gel can be altered. These highly specialized gel filtration and chromatographic media are composed of macroscopic beads synthetically derived from the polysaccharide dextran.
How do I choose a gel filtration column?
Sample Purity Yes, you need to consider their sizes and relative amounts as well before picking your column. Ideally, choose a column that gives the best possible resolution. In case there is more than one option available, choose a column where the component of interest elutes earlier.
What is FPLC system?
The Grotewold lab now has a Fast protein liquid chromatography (FPLC) system. The FPLC allows the user to separate and purify proteins. FPLC generally uses a higher flow rate and lower pressures to move the buffers and sample through the separating column.
Why is blue dextran used in gel filtration chromatography?
Blue dextran is used to calculate void volume in gel filtration chromatography, but it is expensive. So I want to use any cheaper alternative for this.
How can I improve my gel filtration?
Increase in column length increases the resolution and increase in column diameter results in high bed volume and hence higher column capacity. The fractionation range and the exclusion limit can be controlled by varying pore size. The smaller the particle size of the gel, the higher the resolution achieved.
Is gel filtration the same as size exclusion?
Gel filtration chromatography, also known as size exclusion chromatography, is used to separate molecules of different sizes. In addition to separating different proteins of varying size, one may resolve oligomeric forms of a particular protein.
What is blue dextran?
Blue Dextran is a high-molecular-weight glucose polymer (original mol wt 2 x 10(6) g/mol) containing covalently bonded Reactive Blue 2 dye (approximately mmol/g dextran). This blue dye is known for its high binding affinity to a wide variety of proteins, with a particularly high affinity for serum albumin.
How much does an FPLC cost?
Marshall Scientific’s Recertification Protocol
| GE AKTA CU-950 Control Box | GE AKTA pH/C-900 Monitor | GE AKTA Purifier 100 FPLC System w/ UPC-900 |
|---|---|---|
| GE AKTA Frac-950 Fraction Collector | GE AKTA UPC-900 Monitor | GE AKTA Purifier 100 FPLC System w/ UV-900 Detector |
| Sale Price: $3,165.00 | Sale Price: $3,675.00 | Sale Price: $18,885.00 |
How does an FPLC work?
Fast protein liquid chromatography (FPLC) is a form of medium-pressure chromatography that uses a pump to control the speed at which the mobile phase passes through the stationary phase. Since then, many different medium-pressure chromatography systems have been developed.
What is Superose 6 10/300 GL used for?
Superose 6 10/300 GL is a prepacked column for high-performance size exclusion chromatography separation and analysis within the broad fractionation range for molecules between 5000 to 5 000 000. Superose 6, 10/300 GL (17517201) is discontinued and replaced by Superose 6 Increase 10/300 GL (29091596)
What is Superose 6 increase?
Superose 6 Increase is a next-generation, agarose-based matrix for size exclusion chromatography. With smaller and more rigid beads than their predecessors, Superose 6 Increase columns deliver higher resolution protein purification in shorter runtimes.
Why use supersuperose 6 increase 5/150 GL column?
Superose 6 Increase 5/150 GL is for rapid size analysis of proteins and other biomolecules. Short cycle time, together with small sample volume and low consumption of buffer make this column ideal for use in screening experiments to check protein homogeneity.
What is the optimal fractionation range of Superdex 200 increase?
The optimal fractionation range of Superdex 200 Increase is complementary, providing higher resolution for proteins with molecular weights below M r ~ 400 000. This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures.
