How does DiOC6 stain mitochondria?
How does DiOC6 stain mitochondria?
DiOC6 (3,3′-dihexyloxacarbocyanine iodide) is a fluorescent dye used for the staining of a cell’s endoplasmic reticulum, vesicle membranes and mitochondria. Binding to these structures occurs via the dye’s hydrophilic groups. When exposed to blue light, the dye fluoresces green.
Why is the mitochondrial membrane important?
What Is the Purpose of a Mitochondrial Membranes? The inner membrane is also loaded with proteins involved in electron transport and ATP synthesis. This membrane surrounds the mitochondrial matrix, where the citric acid cycle produces the electrons that travel from one protein complex to the next in the inner membrane.
Why is mitochondrial inner membrane impermeable?
Otherwise, the inner membrane is impermeable to most ions and small molecules—a property critical to maintaining the proton gradient that drives oxidative phosphorylation. This is because it contains proteins called porins, which form channels that allow the free diffusion of molecules smaller than about 6000 daltons.
Can you have two fathers?
It is possible for twins to have different fathers in a phenomenon called heteropaternal superfecundation, which occurs when two of a woman’s eggs are fertilized by sperm from two different men. In this case, the woman released multiple eggs within a week of one another.
What is the purpose of the outer membrane?
The outer membrane has several important functions. It acts as a permeability barrier to solutes; it hinders the entry of some antibiotics and protects the cell wall peptidoglycan from lysozyme, which can degrade peptidoglycan, leading to cell lysis.
Why are mitochondria impermeable to protons?
What is mitochondrial membrane permeability?
The mitochondrial inner and outer membranes have contrasting permeability characteristics. The outer membrane is non-specifically permeable to all low-molecular-weight solutes, whereas the inner membrane is impermeable except through specific transporters.
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