How do you identify an unknown bacteria in microbiology?
How do you identify an unknown bacteria in microbiology?
If you have an unknown bacteria and you want to identify it, you’ll typically perform a gram stain and then observe the colony appearance and the individual features. At that point, you can say you have, for example, a gram-negative, aerobic streptobacilli.
What is the purpose of unknown lab report?
The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of …
How can you identify an unknown bacterial culture?
Various steps involved in the identification of unknown bacteria are:
- Isolation:
- Staining Reactions:
- Biochemical reactions:
- Indole test:
- Methyl Red Test:
- Voges Proskauer Test:
- Citrate Utilization Test:
- TSI:
What does a microbiology lab report look like?
The sections required vary from laboratory to laboratory but the standard outline for most lab reports in the biological science include: title, your name, purpose of the experiment, methods, results, discussion and conclusion, references.
How do laboratories identify unknown bacteria?
Identification of unknown bacteria is an integral module in most introductory Microbiology laboratory courses. This laboratory activity typically involves identifying bacteria based on Gram staining for morphology and gram reaction followed by studying their biochemical characteristics.
How do biochemical tests identify unknown bacteria?
Identifying bacteria via analyzing their enzymatic profile Each species of bacteria has specific metabolic needs and relies on different enzymes to fuel those unique needs. The presence of catalase, gelatinase, oxidase, urease, for example, can be used to identify the species of bacteria.
What is an example of a lab report in microbiology?
Example of Unknown Lab Report, Microbiology. The quadrant streak helped spread out the bacteria and made it easier for the two different bacteria to make colonies that could be differentiated by the naked eye. This nutrient agar was incubated at 37° C for two days. The results were checked to see if two separate bacteria were growing.
How do you find the unknowns in a bacterial culture?
The first step to figuring out the unknowns, was to separate the two bacteria. In order to do this, a nutrient agar plate was used. The streak method was used to spread the bacteria across the nutrient agar in hopes of isolating a pure culture of one of the bacteria.
How were multiple procedures practiced in the microbiology lab?
Multiple procedures were practiced in the Microbiology lab to help students better understand how bacteria thrive, grow, and regenerate. These methods were used to determine which two unknown bacteria were present in the test tube. The professor assigned the Unknown 109 test tube.
How do you identify bacteria on MacConkey agar?
Blood Agar (BA) to determine the hemolytic properties of both unknown bacteria MacConkey Agar (MAC) that will select for the gram-negative bacterium and also determine whether or not it is a lactose fermenter. Colonies produced on this plate will also be used for biochemical testing to determine its identification.
