How is next-generation sequencing performed?
How is next-generation sequencing performed?
The basic next-generation sequencing process involves fragmenting DNA/RNA into multiple pieces, adding adapters, sequencing the libraries, and reassembling them to form a genomic sequence. In principle, the concept is similar to capillary electrophoresis.
What is Illumina sequencing?
The Illumina next-generation sequencing (NGS) method is based on sequencing-by-synthesis (SBS), and reversible dye-terminators that enable the identification of single bases as they are introduced into DNA strands. Through ultrasonic fragmentation, the genomic DNA becomes DNA fragment with 200-500bp in length.
What is an Illumina adapter?
Adapters contain: Illumina offers a wide range of adapter kits to allow flexibility and multiple indexing strategies. Illumina adapter kits differ from one another based on the number of samples they support, the length and sequences of the indexes, as well as the chemistry by which they attach to the insert fragment.
Which database is used for next generation sequencing?
Overview of the Clinical NGS database. This database software was developed for the unified management of the detailed clinical information of each patient and next‐generation sequencing analysis results.
Who invented next generation sequencing?
Nick McCooke led the pioneer team at Solexa that invented next-generation sequencing, a technology to read DNA at high speed that is nowadays used worldwide and has laid the foundation for precision medicine.
How does pyrosequencing differ from dideoxy approach?
The main difference between Sanger sequencing and pyrosequencing is that Sanger sequencing is a DNA sequencing approach that uses the dideoxy chain termination method, whereas pyrosequencing is a DNA sequencing approach based on the sequencing-by-synthesis principle.
Is there a suitable method for batch-effect removal from scRNA-Seq data?
With continued growth expected in scRNA-seq data, achieving effective batch integration with available computational resources is crucial. Here, we perform an in-depth benchmark study on available batch correction methods to determine the most suitable method for batch-effect removal.
What is the best way to batch correct single-cell RNA-Seq data?
Batch effects can be highly nonlinear, making it difficult to correctly align different datasets while preserving key biological variations. To address these challenges, tools developed for microarray data batch correction such as ComBat [ 1] and limma [ 2] have been employed on single-cell RNA-seq (scRNA-seq) data.
What is sequencing batch reactor?
The sequencing batch reactor (SBR) is a fill and draw type modified activated sludge process, where four basic steps of filling, aeration, settling and decantation take place sequentially in a batch reactor. Nilesh Tantak, Pavan Raina, in Industrial Wastewater Treatment, Recycling and Reuse, 2014
