What does Janus green stain?
What does Janus green stain?
It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent.
What is the role of Janus Green B stain in this slide preparation?
Explanation: The Janus Green B is a stain which acts as a indicator. The indicator is used to stain the mitochondria in the living cell. It is also used to stain nucleic acid, chromosomes and biomolecules.
Why does Janus green identify mitochondria?
(d) Janus green is used to stain mitochondria. Janus green act as an indicator and changes colour according to the amount of oxygen present. It oxidizes to blue colour in. presence of oxygen and in its absence changes its colour to pink.
How do you make Janus Green?
To prepare a Janus Green B solution, use a 1% aqueous solution. Add 1 g of Janus Green B to 75 ml of DI water. Dilute to a final volume of 100 ml with DI water.
How does Janus green stain work?
Janus Green B is a basic dye and vital stain used in histology. The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.
What is the meaning of Janus green?
Definition of Janus green : a basic azine dye used especially as a vital biological stain (as for mitochondria)
Why should we use Janus green B solution?
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour.
What is the role of Janus green indicator in anaerobic experiment with yeast?
Answer: The indicator Janus Green B changes colour according to the amount of oxygen present. In the absence of oxygen, the indicator is reduced and changes to a pink colour.
Why do we use Diazine green or Janus Green B to glucose solution in the experiment of anaerobic respiration with yeast?
Diazene Green solution is added to the Glucose solution in anaerobic respiration experiment to check the presence of oxygen in glucose solution.
What is Janus Green B stain used for?
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. The indicator Janus Green B changes colour according to the amount of oxygen present.
How does the indicator Janus Green B change colour?
The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour. ^ Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula.
What is the green stain used for in Elisa?
Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622). Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain.