What is retro-orbital bleeding?
What is retro-orbital bleeding?
In retrobulbar bleeding (RBB), also known as retro-orbital bleeding, a capillary tube is used to disrupt the retrobulbar venous sinus located behind the eye. This technique can be used to collect a large volume of blood with or without anesthesia, typically without detriment to the subject animal’s general health.
How do you stop a rat’s tail from bleeding?
Monitor tail tip for re-bleeding and if none seen, clean any blood of tail with gauze and warm water and return rat to its cage. If re-bleeding is seen, apply gentle pressure to tail tip again until bleeding has stopped.
Where is the saphenous vein on a rat located?
Blood is collected from the lateral saphenous vein which runs dorsally and then laterally over the tarsal joint. To improve visualisation of the vein the sample site can be shaved with electric clippers.
What is the anatomical structure in the eye area that is punctured to obtain blood during retro-orbital blood collection?
The tip of the capillary tube is placed at the medial canthus of the eye under the nictitating membrane. A short thrust past the eyeball will enter the slightly resistant membrane of the sinus. The eyeball itself remains uninjured. As soon as the sinus is punctured, blood enters the tubing by capillary action.
How much blood can you draw from a rat?
Single sample: Without fluid replacement, the maximum blood volume which can be safely removed for a one-time sample is 10% of the total blood volume or 5.5-7 ml/kg. With fluid replacement (LRS or sterile physiologic saline), up to 15% of the total blood volume or approximately 8.3-10.5 ml/kg can be removed.
How do you pull blood out of a rat?
Puncture the vein using a 20G needle and scoop the blood as it comes out using a microvette. Pumping with the leg will help draw more blood. When you have collected enough blood, hold a clean compress on the puncture site to stop bleeding.
What is rat vein?
Arsenic trioxide, a poisonous substance. Common wintergreen, Pyrola minor, also known as Rat’s vein.
Do rats blood coagulate?
The rate of intrinsic coagulation reaction was rapid, and plasma coagulation appeared to be of primary importance while the influence of platelet aggregation was minor. A simple model of rat haemostatic mechanism is proposed based on these results.
