What is the indicator for protein How does it work?
What is the indicator for protein How does it work?
Biuret solution is used to identify the presence of protein. Biuret reagent is a blue solution that, when it reacts with protein, will change color to pink-purple.
What is protein level in urine?
Normal amount of protein in the urine are less than 150mg/day. High levels of protein in the urine are associated with rapid decline in kidney function. It affects about 6.7 percent of the United States population.
What is the principle involved in the reagent strip method of determining urine protein?
The reagent strip protein method is based on the principle of “protein-error-of-indicators.” It produces a visible colorimetric reaction that is capable of detecting most instances of proteinuria. Falsely elevated results can occur if the urine sample is visibly bloody.
What is a protein error?
Testing for protein is based on the phenomenon called the “Protein Error of Indicators” (ability of protein to alter the color of some acid-base indicators without altering the pH). In a solution void of protein, tetrabromphenol blue, buffered at a pH of 3, is yellow.
What are the complications of proteinuria?
Complications of proteinuria include the following:
- Pulmonary edema due to fluid overload.
- Acute kidney injury due to intravascular depletion and progressive kidney disease.
- Increased risk of bacterial infection, including spontaneous bacterial peritonitis.
What are the different identification tests for proteins?
Observation and Inference
| Test | Observation |
|---|---|
| Biuret test | The appearance of bluish- violet color confirms the presence of Proteins. |
| Xanthoproteic test | The appearance of yellow color confirms the presence of proteins. |
| Millions test | The appearance of brick red color confirms the presence of proteins. |
What can cause errors in reagent strip testing?
Interferences: If excess urine is left on the reagent strip, a phenomenon known as runover may occur. The urine from one reagent area carries reagent onto the pH test area and changes the result erroneously. Notes: In animals, usually carnivores have more acid pH versus herbivores with more alkaline pH.
Which of the following can give a false negative urine protein reading?
False negative results can be caused by high concentrations of ascorbic acid (vitamin C), albumin or other proteins (>500mg/dL), glucose (>3000 mg/dL), or ketones. Urine with high specific gravity can cause a false negative reaction because enzyme is not as readily released from crenated white blood cells.
What is the protein error of indicators principle?
It is a tradition method of testing protein in reagent strips. • Protein error of indicators principle is colorimetric reaction. • Normally in response to specific pH range, indicators produces particular color, in contrary to this, in the constant pH also, indicators change color when protein exist.
How do you test for protein error?
Testing for protein is based on the phenomenon called the “Protein Error of Indicators” (ability of protein to alter the color of some acid-base indicators without altering the pH). In a solution void of protein, tetrabromphenol blue, buffered at a pH of 3, is yellow.
Is there a protein error in pH determinations?
The so-called “protein-error” in pH determinations with certain dyes used as indicators has been studied; its dependence on the nature of the protein is illustrated by experiments with bromophenol blue (5.5 mg/100 ml of sÖrensen’S citrate-hydrochloric acid buffer, pH 3.5).
What is the color of protein absence at pH 3?
• At pH 3, both of the indicators give yellow color in protein absence. • When concentration of protein increases color change from green —> blue appears. • Results are reported as negative or trace or 1+, 2+, 3+ and 4+ (or) 2000, 300, 100 and 30 mg/dL (Milli grams per deciliter).
