Which of there is the culture medium for Mycobacterium tuberculosis?
Which of there is the culture medium for Mycobacterium tuberculosis?
Mycobacterium tuberculosis is a slow-growing bacterium that is the etiological agent of tuberculosis. Agar-based and egg-based media incorporating green malachite and Middlebrook broths or solid media are recommended as the “gold standard” for isolation, culture, and definite diagnosis of M. tuberculosis (6).
What are the microbial properties of Mycobacterium tuberculosis?
Mycobacteria, such as M tuberculosis, are aerobic, non–spore-forming, nonmotile, facultative, curved intracellular rods measuring 0.2-0.5 μm by 2-4 μm.
How is Mycobacterium tuberculosis culture?
Mycobacterial culture can be performed on either a solid or a liquid medium. The yield of M. tuberculosis isolated from a liquid medium (e.g., Middlebrook 7H9) is greater than that from a solid egg-based medium (e.g., Lowenstein-Jensen [LJ]) or a solid agar-based medium (e.g., Middlebrook 7H11) (17).
What is mycobacteria culture?
Mycobacterial culture is a test to look for the bacteria that cause tuberculosis and other infections caused by similar bacteria.
What are the characteristics of Mycobacterium smegmatis?
Mycobacterium smegmatis is a Gram-positive bacteria, characterized by an inner cell membrane and a thick cell wall. The Gram-positive bacteria is further classified as one with a high GC content and therefore a low AT content. This quality is used as a crude measure of similarity of different species of bacteria.
What is the arrangement of M smegmatis?
Mycobacterium smegmatis str. MC2 155
| Names | Mycobacterium smegmatis str. MC2 155 |
|---|---|
| Cell arrangement | Singles |
| Sporulation | Nonsporulating |
| Metabolism | NA |
| Energy source | Chemoorganotroph |
What are the characteristics of mycobacteria?
Mycobacteria are immobile, slow-growing rod-shaped, gram-positive bacteria with high genomic G+C content (61-71%). Due to their special staining characteristics under the microscope, which is mediated by mycolic acid in the cell wall, they are called acid-fast. This is also the reason for the hardiness of mycobacteria.
What is the morphology of Mycobacterium tuberculosis?
Morphology The Mycobacterium tuberculosisIt is a bacterium that belongs to the group of bacilli. They are rod-shaped, and can be straight or slightly curved cells. They are extremely small cells, measuring approximately 0.5 microns wide by 3 microns long.
What media is used to culture Mycobacterium tuberculosis?
Culturing Mycobacterium tuberculosis can therefore be carried using any of these media: either the liquid media or solid media. Bacteria that are not acid fast when stained absorb the blue color of counter stain but this does not occur in acid fast bacteria.
What is the optimum pH for growth of Mycobacterium tuberculosis?
DISCUSSION. Previous studies investigating the sensitivity of mycobacteria to acid pH have reported growth at a broad range of pH values ( 7, 18 ). These studies reported that the optimum growth of M. tuberculosis in Dubos medium was between pH 5.8 and pH 6.5, with growth observed at as low as pH 5.4 ( 18 ).
What is the procedure for staining Mycobacterium tuberculosis?
The procedure for staining mycobacterium tuberculosis involves taking of sample such as a smear of sputum and covering it with a red stain known as carbolfuchsin. This is then heated in order to help the penetration of the added dye.
